Appunti VERIFICATO
4th Lecture Arbizzani
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- Amperometric biosensors work under non-equilibrium conditions, requiring more sophisticated techniques and instruments for signal measurement.
- The Clark cell, originally used for detecting O2, was adapted into a glucose biosensor by covering its membrane with glucose oxidase; this sensor measures the produced oxygen's electro-chemical potential proportional to glucose concentration.
- In amperometric cells, three electrodes are used: working, auxiliary (counter), and reference. The reference electrode's constant reaction over time can lead to wear out, necessitating an auxiliary electrode for current flow.