Domande d'esame VERIFICATO
Esempio di Compitino di Biologia Molecolare
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- DNA Structure & Function:
- Calculation of double helix turns in DNA (e.g., 120 turns for 1200 bp).
- Explanation of why supercoiled DNA migrates faster in agarose gels.
- Determining the complementary DNA strand.
- Application of Chargaff's rules to calculate nucleotide percentages (e.g., 18% C if A is 32%).
- Classification of Thymine as a pyrimidine.
- Definition of DNA melting temperature (Tm) and its determinants.
- Molecular Techniques:
- Interpretation of Sanger sequencing gel results to determine DNA sequence.
- Calculation of agarose required for gel preparation (e.g., 1.5g for 150ml of 1% gel).
- Components for Sanger sequencing: one dideoxynucleotide and all four deoxynucleotides per tube.
- Understanding RT/PCR and its use in cDNA synthesis.
- Construction of restriction maps from fragment sizes of single and double digests.
- Cloning & Genetic Engineering:
- Description of cosmids, their use in DNA cloning, packaging, and bacterial introduction.
- Mechanism of blue/white screening with X-gal/IPTG; conditions for blue color (intact alpha-peptide coding sequence).
- Explanation of phage infection restriction by bacterial modification patterns.
- Calculation of the minimum number of clones for a genomic library.
- Role of aminoacyl-tRNA synthetase as the "adaptor" in genetic information flow.
- Specificity of aminoacyl-tRNA synthetases in recognizing correct tRNAs.
- Sequence Analysis:
- Method for calculating percentage identity between two DNA sequences.
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