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Fermentation biotech-industrial bioprocesses
Università degli Studi di Milano biotechnology for the bioeconomy - biotecnologia per la bioeconomia 2021
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- Fermentation biotechnology involves converting natural substrates into products using enzymes, either isolated or within cells.
- Key factors for large-scale bioprocesses include controlled genotypes, chemically defined media, cultivation conditions, and operating modes like batch, fed-batch, or continuous.
- CSTR (Continuously Stirred Tank Reactor) bioreactors use mechanical stirring to cause shear stress on cells, while pneumatic bioreactors use air bubbles for gentle mixing; both have specific requirements and limitations regarding oxygen transfer and foam control.
- Process improvement through statistics includes sequential or full factorial designs and Plackett-Burman design to optimize parameters like temperature, pH, medium composition, and nutrient levels, reducing the number of experiments needed.
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